Biochem/physiol Actions

Reversible: no

Primary TargetInhibits cell growth and induces apoptosis in HL-60 cells

Product does not compete with ATP.

Cell permeable: yes

General description

Biologically active, cell-permeable, non-physiological ceramide analog. Inhibits cell growth and induces apoptosis of HL-60 cells. Induces intranucleosomal DNA fragmentation. An activator of heterotrimeric protein phosphatase 2A. Stimulates a cytosolic serine/threonine protein phosphatase in T9 cells at concentrations as low as 100 nM. Activates stress-activated protein (SAP) kinase in HL-60 human promyelocytic cells.

Biologically active, cell-permeable, non-physiological ceramide analog. Inhibits cell growth and induces apoptosis in HL-60 cells. Induces intranucleosomal DNA fragmentation. An activator of heterotrimeric protein phosphatase 2A (PP2A). Stimulates a cytosolic serine/threonine protein phosphatase in T9 cells at concentrations as low as 100 nM. Activates stress activated protein kinase (SAPK) in HL-60 human promyelocytic cells.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Westwick, J.K., et al. 1995. J. Biol. Chem.270, 22689.Cifone, M.G., et al. 1994. J. Exp. Med.180, 1547.Hannun, Y.A. 1994. J. Biol. Chem. 269, 3125.Bielawska, A., et al. 1993. J. Biol. Chem.268, 26226.Obeid, L.M., et al. 1993. Science259, 1769.Ballou, L.R., et al. 1992. J. Biol. Chem.267, 20044.Dobrowsky, R.T., and Hannun, Y.A. 1992. J. Biol. Chem.267, 5048.

Packaging

5 mg in Plastic ampoule

Preparation Note

When diluting into aqueous solution, sonication may be necessary to achieve a uniform suspension.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). DMSO stock solutions are stable for up to 1 month at -20°C. Alcohol stock solutions are stable for ﹤1 week at -20°C.

Warning

Toxicity: Irritant (B)