Biochem/physiol Actions
Reversible: no
Primary TargetInhibits cell growth and induces apoptosis in HL-60 cells
Product does not compete with ATP.
Cell permeable: yes
General description
Biologically active, cell-permeable, non-physiological ceramide analog. Inhibits cell growth and induces apoptosis of HL-60 cells. Induces intranucleosomal DNA fragmentation. An activator of heterotrimeric protein phosphatase 2A. Stimulates a cytosolic serine/threonine protein phosphatase in T9 cells at concentrations as low as 100 nM. Activates stress-activated protein (SAP) kinase in HL-60 human promyelocytic cells.
Biologically active, cell-permeable, non-physiological ceramide analog. Inhibits cell growth and induces apoptosis in HL-60 cells. Induces intranucleosomal DNA fragmentation. An activator of heterotrimeric protein phosphatase 2A (PP2A). Stimulates a cytosolic serine/threonine protein phosphatase in T9 cells at concentrations as low as 100 nM. Activates stress activated protein kinase (SAPK) in HL-60 human promyelocytic cells.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes
Westwick, J.K., et al. 1995. J. Biol. Chem.270, 22689.Cifone, M.G., et al. 1994. J. Exp. Med.180, 1547.Hannun, Y.A. 1994. J. Biol. Chem. 269, 3125.Bielawska, A., et al. 1993. J. Biol. Chem.268, 26226.Obeid, L.M., et al. 1993. Science259, 1769.Ballou, L.R., et al. 1992. J. Biol. Chem.267, 20044.Dobrowsky, R.T., and Hannun, Y.A. 1992. J. Biol. Chem.267, 5048.
Packaging
5 mg in Plastic ampoule
Preparation Note
When diluting into aqueous solution, sonication may be necessary to achieve a uniform suspension.
Reconstitution
Following reconstitution, aliquot and freeze (-20°C). DMSO stock solutions are stable for up to 1 month at -20°C. Alcohol stock solutions are stable for ﹤1 week at -20°C.
Warning
Toxicity: Irritant (B)
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